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KMID : 0043320060290080707
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Archives of Pharmacal Research
2006 Volume.29 No. 8 p.707 ~ p.711
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Effect of Additives on the Viability of Bifidobacteria Loaded in Alginate Poly-l-lysine Microparticles during the Freeze-drying Process
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Cui Jing-Hao
Cao Qing-Ri
Choi Yun-Jaie
Lee Kyung-Hoon
Lee Beom-Jin
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Abstract
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Bifidobacteria-loaded alginate poly-l-lysine microparticles (bap microparticles) were prepared using an air atomization method and then freeze-dried. The viability of the bap microparticles was investigated as a function of the amount of the bifidobacteria cultures, and the addition of a yeast extract, cryoprotectants, antioxidants and neutralizer. The size of the bap microparticles with and without the bifidobacteria was 84.8±28.5 μm (mean±standard deviation) and 113.1±38.5 μm, respectively. The surface morphology was slightly ellipsoid and wrinkled regardless of the incorporating bifidobacteria. The viability gradually decreased with increasing freeze-drying time. Free-flowing powdered bap microparticles were obtained at least 12 h after freeze-drying the wetted slurry of bap microparticles. However, the particles tended to aggregate when either lactose or ascorbic acid was added. The addition of a yeast extract, cryoprotectants (glycerol and lactose), antioxidants (NaHSO3 and ascorbic acid) and neutralizer (Mg3(PO4)2) resulted in a significantly higher viability of the bifidobacteria in the bap microparticles after freeze-drying (0.34-1.84 log) compared with the culture alone.
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KEYWORD
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Additive, Air atomization, Alginate microparticles, Bifidobacteria, Freeze-drying, Viability
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